Jul 01 2013Highlights A novel potent selective inhibitor of human rat and mouse sEH GSK2256294A is described In vitro GSK2256294A exhibits concentration-dependent inhibition of EETs metabolism in whole blood In vivo administration of GSK2256294A results in an increased LTX/LTX diol ratio in rat plasma GSK2256294A inhibits the initiation and/or maintenance of cigarette smoke Oct 01 2008This possibility leads us to the two papers in this issue (Singh et al 2008 Xu et al 2008) that report the identification of a novel BLM complex-associated protein that has been called RMI2 The protein was found to be present in BLM or RMI1 immunoprecipitates and was subsequently identified as a 15 8-kDa polypeptide by mass spectrometry

International Journal of Cancer Management

1 Context A pelvic mass is an enlargement or swelling in the pelvic region (1-5) The most common type of pelvic masses is ovarian masses which included cysts and tumors () Pelvic masses are a prevalent cause of referral to gynecologic oncology departments to evaluate the possibility of benign or malignant conditions () Pelvic mass often was found in pelvic examinations among females with

Soluble epoxide hydrolase (sEH encoded by EPHX2) is the key enzyme involved in EET signaling and hydrolyzes the epoxide moiety to convert EETs to 1 2-diols dihydroxyeicosatrienoic acids (DHETs) that have a lower bioactivity However sEH activity is dependent on oligomerization

Mar 01 2018The list of the identified dysregulated proteins in AD (Additional file 2: Table S2) includes a number of proteins that have been previously shown by our group and others to be differentially expressed in AD brain such as DJ-1 APOE clusterin (CLU) and UCH-L1 [1 17 19 55] In addition our proteomic analysis also identified 322 novel proteins that have not been previously reported as

Mutations in BLM a RecQ-like helicase are linked to the autosomal recessive cancer-prone disorder Bloom's syndrome BLM associates with topoisomerase (Topo) IIIα RMI1 and RMI2 to form the BLM complex that is essential for genome stability The RMI1-RMI2 heterodimer stimulates the dissolution of double Holliday junction into non-crossover recombinants mediated by BLM-Topo IIIα and is

Cigarette Smoke-Induced Pulmonary Inflammation and Autophagy Are Attenuated in Ephx2-Deficient Mice Yunxiao Li 1 Ganggang Yu 1 Shaopeng Yuan 0 Chunting Tan 1 Puqiao Lian 0 Lixia Fu 0 Qi Hou 0 Bo Xu 1 Haoyan Wang 1 0 Beijing Key Laboratory of New Drug Mechanisms and Pharmacological Evaluation Study Institute of Materia Medica Chinese Academy of Medical Sciences and Peking

Research Paper Identification of breast cancer candidate

treatment targets Here we used subnetwork extraction algorithms to identify novel BC related genes based on the known BC genes (seed genes) gene co-expression profiles and protein-protein interaction network We computationally predicted seven key genes (EPHX2 GHRH PPYR1 ALPP KNG1 GSK3A and TRIT1) as putative genes of BC

The role of altered redox status and high reactive oxygen species (ROS) is still controversial in cancer development and progression Intracellular levels of ROS are elevated in cancer cells suggesting a role in cancer initiation and progression on the contrary ROS elevated levels may induce programmed cell death and have been associated with cancer suppression Thus it is crucial to

Feb 09 2018Sato et al (2004) demonstrated a significant modification of the phenotype of familial hypercholesterolemia (see 143890) resulting from a defective allele at the LDLR locus (606945 0063) by the arg287 variation in the EPHX2 gene (132811 0001) Fornage et al (2005) genotyped 12 SNPs in the EPHX2 gene in 315 stroke patients and 1 021 controls from the ARIC study and identified 2 common EPHX2

Feb 09 2018Sato et al (2004) demonstrated a significant modification of the phenotype of familial hypercholesterolemia (see 143890) resulting from a defective allele at the LDLR locus (606945 0063) by the arg287 variation in the EPHX2 gene (132811 0001) Fornage et al (2005) genotyped 12 SNPs in the EPHX2 gene in 315 stroke patients and 1 021 controls from the ARIC study and identified 2 common EPHX2

Mar 20 2012The RMI subcomplex (RMI1/RMI2) functions with the BLM helicase and topoisomerase IIIα in a complex called the "dissolvasome " which separates double-Holliday junction DNA structures that can arise during DNA repair This activity suppresses potentially harmful sister chromatid exchange (SCE) events in wild-type cells but not in cells derived from Bloom syndrome patients with inactivating

Soluble epoxide hydrolase (sEH encoded by EPHX2) is the key enzyme involved in EET signaling and hydrolyzes the epoxide moiety to convert EETs to 1 2-diols dihydroxyeicosatrienoic acids (DHETs) that have a lower bioactivity However sEH activity is dependent on oligomerization

The initial finding linking the two complexes was identification of an ICL-induced supercomplex called BRAFT (BLM coupled with the strong effects of the RMI2 Lys121Ala variant on complex formation argue that this interaction is a key anchor point in the RMI1/RMI2/FANCM ternary complex et al BLAP18/RMI2 a novel OB-fold-containing

Jun 08 2020Liu J Nie S Gao M Jiang Y Wan Y Ma X Zhou S Cheng W Identification of EPHX2 and RMI2 as two novel key genes in cervical squamous cell carcinoma by an integrated bioinformatic analysis J Cell Physiol 2019 234(11):21260–73 CAS PubMed Article Google Scholar

Proteomics and bioinformatics analysis of mouse

The aim of this study was to identify differently expressed proteins in the presence and absence of EPHX2 gene in mouse hypothalamus using proteomics profiling and bioinformatics analysis This study was performed on 3 wild type (WT) and 3 EPHX2 gene global knockout (KO) mice (EPHX2-/-) Using the nano- electrospray ionization (ESI)-LC-MS/MS detector we identified 31 over-expressed proteins in

Sep 01 2018In INFORM the overall 5-year mortality rate was 17 1% An increase in 5-year mortality rates was observed across EPHX2 p Lys55Arg genotype groups and Arg55 variant allele carriers exhibited a significantly higher risk of death (22 4% in Arg55 carriers versus 15 3% for Lys/Lys unadjusted HR 1 50 95% confidence interval [CI] 1 02–2 22 P = 0 042) (Fig 1A Supplemental Table 3)

The form of sEH in the intracellular environment is a homodimer with two distinct activities in two separate structural domains of each monomer: the C-terminal epoxide hydrolase activity (soluble epoxide hydrolase: EC 3 3 2 10) and the N-terminal phosphatase activity (lipid-phosphate phosphatase: EC 3 1 3 76) sEH converts epoxides or three membered cyclic ethers to their corresponding diols

Feb 01 2011The results are presented as means SD Statistical analyses were performed using Student's t-test (P 0 05 P 0 01 and P 0 001) and Pearson's correlation coefficient unless otherwise indicated Immunohistochemistry Acetone-fixed frozen sections (6 μm) of primary prostate cancer samples were dried and endogenous peroxidase activity was blocked using 0 3% hydrogen peroxide (H 2 O 2)

Essential component of the RMI complex a complex that plays an important role in the processing of homologous recombination intermediates It is required to regulate sister chromatid segregation and to limit DNA crossover Essential for the stability localization and function of BLM TOP3A and complexes containing BLM In the RMI complex it is required to target BLM to chromatin and

Interestingly rs2279590 locus has a widespread enhancer effect on two nearby genes protein tyrosine kinase 2 beta (PTK2B) and epoxide hydrolase-2 (EPHX2) both of which have been previously associated with AD as risk factors To summarize our study unveils a mechanistic role of the common variant rs2279590 that can affect a variety of aging

Jinhui Liu Sipei Nie Mei Gao Yi Jiang Yicong Wan Xiaoling Ma Shulin Zhou Wenjun Cheng Identification of EPHX2 and RMI2 as two novel key genes in cervical squamous cell carcinoma by an integrated bioinformatic analysis Journal of Cellular Physiology