Rosiglitazone Inhibits Activation of Hepatic Stellate Cells via Up-Regulating Micro-RNA-124-3p to Alleviate Hepatic Fibrosis Digestive Diseases and Sciences doi: 10 1007/s10620-019-5462-8 [HOTAIR expression clone] Cheng G and Liu S-Y (2019) Broad antiviral therapy with "Aqueous extract of Arbutus unedo inhibits STAT1 activation in human breast cancer cell line MDA--231 and human fibroblasts through SHP2 activation " Med Chem 4 219-228 2008 31 "S-Glutathionylation at Cys328 and Cys542 Impairs STAT3 Phosphorylation " ACS Chem Biol 15 1885-93

S Glutathionylation at Cys328 and Cys542 Impairs STAT3

S‑Glutathionylation at Cys328 and Cys542 Impairs STAT3 (HNO) donor inhibits STAT3 phosphorylation through the formation of sulfenic acid at the cysteine residues 24 In this regard other reports demonstrated that protein sulfenic acids are intermediate stage in the oxidation of cysteine and are able to react with

The microRNA miR-34a inhibits prostate cancer stem cells and metastasis by directly repressing CD44 Nat Med 2011 17(2):211‒5 doi: 10 1038/nm 2284 Saleh AD Savage JE Cao L Soule BP Ly D DeGraff W et al Cellular stress induced alterations in microRNA let-7a and let-7b expression are dependent on p53

Thaiparambil JT Bender L Ganesh T Kline E Patel P Liu Y Tighiouart M Vertino PM Harvey RD Garcia A Marcus AI Withaferin A inhibits breast cancer invasion and metastasis at sub-cytotoxic doses by inducing vimentin disassembly and serine 56 phosphorylation Int J Cancer 2011 Dec 1 129(11):2744-55 doi: 10 1002/ijc 25938 Epub 2011 May 2

S-Glutathionylation is the specific post-translational modification of protein cysteine residues by the addition of the tripeptide glutathione the most abundant and important low-molecular-mass thiol within most cell types Protein S-glutathionylation is promoted by oxidative or nitrosative stress but also occurs in unstressed cells It can serve to regulate a variety of cellular processes by

Consistent with our experimental results we found evidence that S-glutathionylation exists in mouse for all the proteins reported in Table 1 including a clear coincidence for the modified Cys328 identified in vimentin with the unique exception of tubulin beta-4B chain which nevertheless was found as S-glutathionylated in humans

Recombinant Human Vimentin(VIM)

Vimentin and Axl expression might contribute to the aggressive phenotype in breast cancer PMID: 27506606 our findings suggest that the HIF-1alpha-HDAC1 complex transcriptionally inhibits miR-548an expression during hypoxia resulting in the upregulation of vimentin that facilitates the pancreatic tumorigenesis PMID: 27353169

Background Metastasis and invasion are the main causes of mortality in gastric cancer To improve the treatment of gastric cancer the development of effective and innovative antitumor agents toward invasion and proliferation is needed Alpha-lipoic acid (ALA) a naturally occurring thiol antioxidant showed antiproliferative and cytotoxic effects on several cancers

Although S-glutathionylation seems to play a key role in cellular (hrTP) human recombinant vimentin (hrVM) and rat collapsin response mediator protein-2 (rCRMP-2)] by (MMP)-9 inductions in RAW 264 7 cells Aimed at evaluating the mechanism of action by which SG inhibits the LPS-mediated induction of MMP-9 the effects of SG on nuclear

Number of items at this level: 134 A Adamczyk Justyna and Bal Wojciech and Krezel Artur (2015) Coordination properties of dithiobutylamine (DTBA) a newly introduced protein disulfide reducing agent Inorganic Chemistry 54 pp 596-606

Jun 25 2007The natural product withaferin A (WFA) exhibits antitumor and antiangiogenesis activity in vivo which results from this drug's potent growth inhibitory activities Here we show that WFA binds to the intermediate filament (IF) protein vimentin by covalently modifying its cysteine residue which is present in the highly conserved α-helical coiled coil 2B domain

Among these 12 major proteins of heterogeneous function showed a significant increase in S-nitrosylation following shear flow The S-nitrosylated residues in tropomyosin and vimentin which were localized in the hydrophobic motif of each protein were identified as Cys170 and Cys328 respectively

S-glutathionylation of caspase-3 by GSNO or SNAP plus GSH has been described [102 104 105] and very recent observations claim that caspase-3 experiences S-glutathionylation on several thiol residues and that this process is intracelullarly regulated by the thiol transferase glutaredoxin by a mechanism that was relevant for the regulation of

Cancer is a complex disorder extremely dependent on its microenvironment and highly regulated by multiple intracellular and extracellular stimuli Studies show that reactive oxygen and nitrogen species (RONS) play key roles in cancer initiation and progression Accumulation of RONS caused by imbalance between RONS generation and activity of antioxidant system (AOS) has been observed in many

Antioxidants

S-glutathionylation inhibits PTP1B activity [33 34] Although the functional consequences of this modification were not studied in directly in ECs mice lacking PTP1B display enhanced angiogenic and revascularization capacities as well as an increased cardiac perfusion following myocardial infarction Further studies are needed to investigate

Jun 01 2007Vimentin-WFA or vimentin alone (0 5 μg) was mixed with the Chariot protein transduction reagent (Active Motif) and incubated for 30 min at 24C to form complex according to the manufacturer's instructions The protein-Chariot complex was subsequently added to BAECs in serum-free medium and incubated for 1 hr in 37C-5% CO 2 conditions

Withania somnifera Root Extract Inhibits Mammary Cancer Metastasis and Epithelial to Mesenchymal Transition Withaferin A Effectively Targets Soluble Vimentin in the Glaucoma Filtration Withaferin A Effectively Targets Soluble Vimentin in the Glaucoma Filtration Surgical Model of Fibrosis

Mar 01 2018Recently we reported that perturbation in the intracellular redox environment induces reversible S-glutathionylation of STAT3 essentially at Cys328 and Cys542 and that this modification prevents phosphorylation of the protein impairing activation of STAT3 signaling in different cell lines On the other hand the implication of S-glutathionylation in the redox regulation of STAT1 has

Vimentin S-glutathionylation at Cys328 inhibits filament elongation and induces severing of mature filaments in vitro April 07 2020 [ MEDLINE Abstract] Reverse protein engineering of a novel 4-domain copper nitrite reductase reveals functional regulation by protein-protein interaction April 07 2020 [ MEDLINE Abstract]

Consistent with our experimental results we found evidence that S-glutathionylation exists in mouse for all the proteins reported in Table 1 including a clear coincidence for the modified Cys328 identified in vimentin with the unique exception of tubulin beta-4B chain which nevertheless was found as S-glutathionylated in humans

Dec 19 2017Vascular smooth muscle cells (VSMCs) are the stromal cells of the vascular wall and are responsible for regulating arterial tone blood pressure and blood supply of the tissues VSMCs display diversity in function and phenotype depending on their location within the arterial tree (large conduit vs small resistance vessels) their embryologic origin and their organ-dependent microenvironment