Salinity is a major abiotic stress that limits plant productivity and quality throughout the world Roots are the sites of salt uptake To better understand salt stress responses in maize we performed a comparative proteomic analysis of seedling roots from the salt-tolerant genotype F63 and the salt-sensitive genotype F35 under 160 mM NaCl treatment for 2 days Mar 29 2019Carrot is an important root vegetable crop with a variety of nutrients As the main product of carrots the growth and development of fleshy roots directly determine the yield and quality of carrots However molecular mechanism underlying the carrot root formation and expansion is still limited In our study isobaric tags for relative and absolute quantification (iTRAQ) was utilized to

iTRAQ

Abstract To investigate the unintended effects of genetically modified (GM) crops an isobaric tags for relative and absolute quantitation (iTRAQ)-based comparative proteomic analysis was performed with seed cotyledons of two GM soybean lines MON87705 and MON87701MON89788 and the corresponding non-transgenic isogenic variety A3525

May 23 2011We used Arabidopsis root tissue as both ABA and G-proteins individually and in combination affect root growth and development We identified 720 proteins of which 42 showed GPA1-dependent and 74 showed ABA-dependent abundance changes iTRAQ Protein Profile Analysis of Tomato Green-ripe Mutant Reveals New Aspects Critical for Fruit Ripening

Feb 04 2013Quantitative differences in protein abundance between wild-type and gtg1gtg2 were analyzed for evaluation of the effect of ABA on the root proteome and its dependence on the presence of functional GTG1/GTG2 proteins The results presented in this study reveal the most comprehensive ABA-responsive root proteome reported to date in Arabidopsis

To investigate the mechanisms underlying Fe homeostasis in plants Fe deficiency-induced changes in the protein profile of Arabidopsis (Arabidopsis thaliana) roots were comprehensively analyzed using iTRAQ (Isobaric Tag for Relative and Absolute Quantification) differential liquid chromatography-tandem mass spectrometry on a LTQ-Orbitrap with

iTRAQ Protein Profile Analysis of Arabidopsis Roots Reveals New Aspects Critical for Iron Homeostasis1[C][W] Ping Lan Wenfeng Li Tuan-Nan Wen Jeng-Yuan Shiau Yu-Ching Wu Wendar Lin and Wolfgang Schmidt* Institute of Plant and Microbial Biology Academia Sinica 115 Taipei Taiwan

iTRAQ

Cytochrome C was linked to apoptosis in the protein profile of TMV-infected plants ferroptosis-like cell death has been reported in Arabidopsis root hairs subjected to 55 20 mM ammonium formate in water pH 10 For downstream LC–ESI–MS/MS analysis iTRAQ labelled peptides were subjected to reverse-phase high performance liquid

Salinity is one of the most serious threats to world agriculture An important sugar-yielding crop sugar beet which shows some tolerance to salt via a mechanism that is poorly understood Proteomics data can provide important clues that can contribute to finally understand this mechanism Differentially abundant proteins (DAPs) in sugar beet under salt stress treatment were identified in

Transcriptional Profiling of the Arabidopsis Iron Deficiency Response Reveals Conserved Transition Metal Homeostasis Networks Plant Physiology 152: 2130-2141 Ping Lan Wenfeng Li Tuan-Nam Wen Jeng-Yuan Shiau Yu-Ching Wu Wen-Dar Lin and Wolfgang Schmidt iTRAQ protein profile analysis of Arabidopsis roots reveals new aspects critical for

LIU Wei-guo WEN Bing-xiao ZHOU Tao WANG Li GAO Yang LI Shu-xian QIN Si-si LIU Jiang YANG Wen-yu 2019 iTRAQ protein profile analysis of soybean stems reveals new aspects critical for lodging in intercropping systems Journal of Integrative Agriculture 18(9): 2029-2040 URL:

Quantification of gene expression in Arabidopsis root hairs Expression of the gene encoding for expansin 7 (EXP7) is tightly linked to root-hair initiation and elongation [] In five-day-old seedlings carrying a construct containing the EXP7 promoter sequence positioned upstream of a green fluorescent protein (GFP) reporter expression of the pEXP7-GFP chimera was observable in developing and

May 16 2008The root of Arabidopsis grows from stem cells at the tip We dissected roots into four longitudinal zones (LZ data set in Fig 1A and table S1) for analysis using the position of cells along the longitudinal axis as a proxy for developmental time (fig S2) () Cell identity varies along the radial axis epidermal cells constitute the outermost tissue followed by the cortex endodermis and

A total of 7491 proteins were identified from Arabidopsis thaliana roots by mass spectrometry 35 2% of which were predicted to contain at least one transmembrane helix Among the quantifiable proteins five were significantly differentially expressed between the

OFFGEL electrophoresis is an established method for proteomic analysis of complex protein samples and was recently applied to the identification of low-abundance proteins and quantitative proteomics by label-free or iTRAQ analysis (Malmstrm et al 2009 Ernoult et al 2010 Warren et al 2010 Zhang et al 2010 Abdallah et al 2012 Sudhir

Nanobody

Feb 27 2020Plant cells perceive and adapt to an ever-changing environment by modifying their plasma membrane (PM) proteome Whereas secretion deposits new integral membrane proteins internalization by endocytosis removes membrane proteins and associated ligands largely with the aid of adaptor protein complexes and the scaffolding molecule clathrin Two adaptor protein complexes function in

Protein Pilot 5 0 (AB Sciex Foster City CA USA) was used for protein quantitation and deep proteome analysis with mgf files as input In detail these parameters were as follows: "Thorough ID" mode with 95% confidence level iTRAQ peptide labeling trypsin digestion and Cys oxidation by methyl methanethiosulfonate (MMTS)

May 14 2019Junliang Li Jie Cui Dayou Cheng Cuihong Dai Tianjiao Liu Congyu Wang Chengfei Luo iTRAQ protein profile analysis of sugar beet under salt stress: different coping mechanisms in leaves and roots BMC Plant Biology 10 1186/s12870-020-02552-8 20 1 (2020)

Sep 27 2017Lan P Li W Wen TN Shiau JY Wu YC Lin W et al (2011) iTRAQ protein profile analysis of Arabidopsis roots reveals new aspects critical for iron homeostasis Plant Physiology 155(2):821–834 pmid:21173025 View Article PubMed/NCBI Google Scholar 25

Transcriptional Profiling of the Arabidopsis Iron Deficiency Response Reveals Conserved Transition Metal Homeostasis Networks Plant Physiology 152: 2130-2141 Ping Lan Wenfeng Li Tuan-Nam Wen Jeng-Yuan Shiau Yu-Ching Wu Wen-Dar Lin and Wolfgang Schmidt iTRAQ protein profile analysis of Arabidopsis roots reveals new aspects critical for

Protein extraction isobaric tags for relative and absolute quantification (iTRAQ) labeling and pooling Total soluble protein was extracted according to the protocol reported by Wang et al (2006) Protein samples were quantified using a CBX protein assay (G‐Biosciences St Louis Missouri USA)

Protein extraction isobaric tags for relative and absolute quantification (iTRAQ) labeling and pooling Total soluble protein was extracted according to the protocol reported by Wang et al (2006) Protein samples were quantified using a CBX protein assay (G‐Biosciences St Louis Missouri USA)

Mar 28 2014Identification of NaCl and NaHCO 3 stress responsive proteins in tomato roots using iTRAQ-based analysis Author links open overlay panel Biao Gong 1 Cunjia Zhang 1 Xiu Li Dan Wen Shuoshuo T -N Wen J Y Shiau Y C Wu W Lin W SchmidtITRAQ protein profile analysis of Arabidopsis roots reveals new aspects critical for iron homeostasis

Mar 22 2011Protein identification from cassava root To combine our large‐scale cassava root proteome analysis with quantitative information on proteins modulated during PPD we used isobaric tags for relative and absolute quantification (iTRAQ) (Ross et al 2004 Zieske 2006)